Application

Research Sub CategoryNuclear Receptors

This Anti-phospho ATM/ATR (Thr1989) Antibody is validated for use in Western Blotting for the detection of phospho ATM/ATR (Thr1989).

Research CategoryEpigenetics & Nuclear Function

Western Blotting Analysis: A representative lot detected ATM/ATR (Thr1989) in ATR TR cells (Nam, E.A, et al. (2011). JBC. 286(33):28707–28714).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The Ataxia-Telangiectasia and Rad3 related kinase (ATR) is a nuclear serine/threonine kinase that is part of the "first-response" system to DNA damage induced by a wide variety of factors including double-stranded breaks and replication stress. The localization of ATR to sites of DNA damage is aided by the Replication Protein A (RPA). Phospho - ATR (Ser428) targets a number of proteins including BRCA1, WRN, CHEK1, MCM2, and p53/TP53, and coordinates signaling pathways involved in DNA repair and apoptosis. ATR may also be involved in the phosphorylation of the histone H2A.X after replication stress, an early marker of DNA damage. However, ATR also mediates DNA replication and fork stability in normal cell cycles by activation of Chk1. Defective ATR contributes to Seckel syndrome type 1.

Immunogen

Linear peptide corresponding to human ATM/ATR (Thr1989).

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in 2mM hydroxyurea treated 293T cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected ATM/ATR (Thr1989) in 10 µg of 2mM hydroxyurea treated 293T cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~300 kDa observed